Joint immobilization of plant growth-promoting bacteria and green microalgae in alginate beads as an experimental model for studying plant-bacterium interactions.
نویسندگان
چکیده
A simple, quantitative experimental model, offering a convenient and basic approach to studies of plant-bacterium interactions, is proposed. This involves immobilizing a unicellular, freshwater microalga, a Chlorella species, serving as the plant, with a plant growth-promoting bacterium, an Azospirillum species, in small alginate beads to allow close interaction and to avoid external interference from bacterial contaminants.
منابع مشابه
Increased growth of the microalga Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense.
Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacterium Azospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigment...
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Coimmobilization of the freshwater microalga Chlorella vulgaris in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense under semi-continuous synthetic wastewater culture conditions significantly increased the removal of ammonium and soluble phosphorus ions compared to immobilization of the microalgae alone. In continuous or batch cultures removal of these ions ...
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Cell-cell interaction in the eukaryote-prokaryote model of the unicellular, freshwater microalga Chlorella vulgaris Beij. and the plant growth-promoting bacterium Azospirillum brasilense, when jointly immobilized in small polymer alginate beads, was evaluated by quantitative fluorescence in situ hybridization (FISH) combined with SEM. This step revealed significant changes, with an increase in ...
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Growth of and the capacity to take up nitrogen in the freshwater microalgae Chlorella vulgaris were studied while varying the concentrations of ammonium and nitrate, the pH and the source of carbon in a synthetic wastewater growth medium when co-immobilized in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense. Analyses of 29 independent experiments showed tha...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 74 21 شماره
صفحات -
تاریخ انتشار 2008